How do you prove Target Engagement in tissue?

Proving target engagement is the cornerstone of validating your drug's mechanism of action, and we use the most rigorous method available to do it in tissue. While standard multiplex IHC services can show that your drug and its target are in the same neighborhood, our specialized isPLA service confirms they are physically interacting.

Using an In Situ Proximity Ligation Assay (isPLA), we generate a specific signal only when two proteins (like your drug and its target) are within nanometers of each other. This powerful technique provides unambiguous, dot-like evidence of true molecular interaction, giving you the highest level of confidence that your drug is hitting its mark in the complex environment of human tissue.