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Whole Slide Imaging

 

Conventional methods used to visualize and evaluate results from histopathology based analyses on tissue sections is typically conducted by visual inspection and photography of the staining results in regular microscopes. Although this can generate excellent data, it is also hampered by a range of significant drawbacks including: slow flow-through, highly work-intensive, challenging to extract and compare data between cases, risk for introduction of data bias when selecting field-of-view, and fading of fluorophores skewing the data


Digital slide scanning is performed with help of robotic microscopes that scan entire tissues stained with chromogenic and fluorescent detection reagents at high speed and resolution (using 20x or 40x objectives). The resulting image files can then be uploaded to dedicated viewing software

 
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Benefits of Digital Slide Scanning

Work-load and flow-through

• Very fast imaging

• Minimized hands-on time


Quality of image acquisition

• No fading of fluorophores during scanning and viewing


Viewing of data and collaboration

• Easy and fast zoom and panning

• Easy switch between multiple fluorophores while viewing,

• Extract more info by simultaneous and synchronized viewing of multiple slides

• Share images digitally for remote viewing

• Fast export of images for presentation


Quantitative analyses
• Acquire data from larger tissue areas
• Minimized risk for introduction bias during image acquisition
• Stronger and more reliable data

 
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Our Services

We scan your slides using a 3D Histech 250 FLASH II digital slide scanner equipped
with a Lumencor Spectra-x LED-lamp light source. It has the capacity to scan slide-mounted
tissue sections:
• At up to 52x (brightfield) and 62x (fluorescence) magnification
• In multiple focal planes and if required collapse the layers for extended focus
• Stained with up to 6 different fluorophores

In more challenging cases we can also image your slides using a ZEISS AxioImager Z1 microscope
equipped with an Apotome. If needed we also acquire images using confocal and STED microscopes