My target is low in abundance, can Offspring still detect it?

Absolutely. We understand that many of the most important drug targets, especially in fields like neuroscience, are not highly expressed. Standard IHC often fails to visualize proteins with low expression levels or overcome IHC background noise, leading to false negatives. We overcome this by employing high-sensitivity signal amplification techniques.

To do this, we utilize advanced signal amplification methodologies like Tyramide Signal Amplification (TSA), which can increase detection sensitivity over 100-fold compared to standard methods. This allows us to confidently perform subcellular localization services and other analyses on even the most challenging targets, providing clear and reliable data where other labs might only find background. We also utilize and specialized probe-based assays like RNAscope™ (an advanced ISH service) for mRNA detection. These powerful techniques allow us to visualize and quantify low-expression targets that are undetectable by other means, ensuring you don't miss a critical finding.