New publication, “Identification and in vitro characterization of C05-01, a PBB3 derivative with improved affinity for alpha-synuclein” together with Karolinska Institutet, Andrea Varrone research group. The approach used in this study also shows that TMAs are a useful tool for the evaluation of ligands in tissue from different proteinopathies, using both fluorescence and autoradiographic assays
https://doi.org/10.1016/j.brainres.2020.147131
The neuropathological hallmark of Parkinson's disease, multiple system atrophy and dementia with Lewy bodies is the accumulation of α-synuclein. The development of an imaging biomarker for α-synuclein is an unmet need. Investigative compound C05-01 binds to both Lewy bodies and Lewy neurites, and displays specific binding in PD and MSA tissue
Fig. (A) Autoradiography (ARG) showing total and non-specific (NS) binding of [3H]C05-01 (1 nM) in tissue from one PD, one control (CO), and one AD case. The NS binding was determined in the presence of cold C05-01 at 10 µM. (B) ARG showing total [3H]C05-01 binding (1 nM) in a PD sample (amygdala, AMY), in different regions of interest. Co-localization of ARG binding and positive immunoreactivity is shown for α-synuclein (α-syn) and amyloid β (aβ) and negative for tau
