In Situ Proximity Ligation Assay
In situ Proximity Ligation Assay (isPLA) is a technology which allow for detection of proteins, protein modifications and protein:protein interactions in immobilized cells or tissue sections. We have extensive experience designing and executing isPLA-based studies.
The technique, which was originally developed in the laboratory of Ulf Landegren at Uppsala University and further developed and marketed by Olink Biosciences as DuoLink, relies on the binding of two different antibodies in close proximity. When this pair of antibodies bind to their protein epitopes within a single protein or within a protein:protein complex, they become substrate for a detection method which pinpoints this molecular event (the proximity ligation assay) with high specificity and sensitivity.
Uses And Applications
• Validation of antibodies for immunohistochemistry and immunocytochemistry
• Highly specific analyses of protein expression and distribution in cells and tissues
• Building target-specific biomarker assays for target engagement studies, recording ligand – triggered protein modifications (eg activity dependent phosphorylations) or protein:protein formations
• Phenotyping the cells that are labelled in the isPLA reaction is achieved by multiplexing this method with conventional immunohistochemistry
•We quantify the results by image analysis, using commercial algorithms and deep learning