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Multiplex Tissue Analysis

Analysis of multiple biomarkers in tissues with preserved anatomical context offers new and increasingly powerful opportunities to extract essential information about the underlying mechanisms that drive various disease processes. Such analyses are therefore gaining strong adoption in clinical research and by teams within the pharmaceutical industry as valuable tools for target validation, efficacy analyses and patient stratification 


These analyses are however demanding to execute as they require investments in an expensive infrastructure, finely calibrated and integrated work-flows and highly trained staff, in particular so when larger cohorts of tissues are to be processed and quantitatively analysed

Offspring offers a unique capability and capacity to co-process large amounts of tissues for analyses of multiple biomarkers, using Nanostring and/or imunofluorescence-based analyses and pre-established or custom-built panels for multiplexed biomarker analysis. We combine that with agile digital scanning of the stained tissue sections and AI-powered quantitative image analysis to generate reliable data for critical stop/go decisions

Multiplex Tissue Analysis: About Us

Offspring's Preestablished Panels

Offspring Biosciences is set up to assist your projects with co-staining of multiple biomarkers in tissue sections using a range of techniques, including up to 5-plexed immunohistofluorescence (IHF), 3-plexed chromogenic immunohistochemistry (IHC) and 2-plexed in situ hybridization histochemistry (ISH). We perform these analyses with pre-established panels for multiplexed biomarker staining or by custom build to flexibly adapt their design to your specific needs.

Listed below are examples of the multiplexed analyses we can run for you:

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CD8 and NK Cell Activity

Granzyme B

This 4-plex-antibody panel enables us to evaluate if your drug triggers cytotoxic T and NK cells infiltration. This can be used in preclinical treatment studies of syngeneic mouse models for cancer. More specifically, the CD8 and NKp46 are markers for cytotoxic T cells and NK cells, respectively. Granzyme B is one of the T and NK cytotoxic cell effector enzymes used to kill cells (eg tumor cells). Ki67 identifies cells in proliferation; for instance, showing if the treatment influences T or NK cells’ proliferation.

CD4 Cell Activity


The CD4 cell activity antibody panel is used to identify and profile the functional state of CD4-positive T cells. For instance, this can be used in the context of mouse syngeneic tumor environments. More specifically, cells marked with only CD4 are identified as T helper cells. When CD4 cells also express FoxP3 they are acting in an immunosuppressive T regulatory state. PD-1 and TIM-3 are both cell surface receptors actively induced on T cells after antigenic stimuli. They can indicate the immunosuppressed/exhausted phenotype of either CD4 or CD8 cells.

Tumor Phenotype and Activity

Activity Marker

This 4-plex antibody panel allows us to state the phenotype and activity of a mouse or human tumor as means to evaluate the effect of a drug in treatment studies. In this panel, antibodies identify the tumor’s epithelial or mesenchymal-like phenotype. In pan-cytokeratin or vimentin positive tumor cells, the nuclear Ki-67 staining “transcriptional activity marker” show their activation and proliferation. Hoescht 33342 nuclear staining also provides information about the viability of the tumor cells. One of two serial sections can be stained with this panel and the results combined with an adjacent immune marker panel revealing the physical/anatomical relation between the tumor cell mass and invading immune cells.

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Parkinson's Disease Pathology

PGP 9.5

Alzheimer's Disease Pathology

Amyloid Beta

Macrophage Subsets

Custom Biomarker



Pancreatic Islet

Secretogranin II




Pancreatic Islet

Pan-Islet Cell Marker

Multiplex Tissue Analysis: Team Members
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